| Description: |
hCG is produced from a sterile preparation of placental glucoprotein urine of post-menopausal women having a total molecular mass of 36,700 Dalton. hCG consists of 237 amino acids, chain-92 amino acids and chain-145 amino acids.
The hCG is purified by proprietary chromatographic techniques. |
| Source: |
Urine of post-menopausal women |
| Physical Appearance: |
Sterile Filtered White lyophilized (freeze-dried) powder.
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| Formulation: |
The hCG was lyophilized from a concentrated (1mg/ml) solution with no additives.
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| Solubility: |
It is recommended to reconstitute the lyophilized hCG in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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| Stability: |
Lyophilized hCG although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution hCG should be stored at 4 C between 2-7 days and for future use below -18 C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
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| Purity: |
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
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| Biological Activity: |
ProSpec's hCG is biologically active compared to standard (IRP reference preparation 75/551).
The activity was found to be 3559IU/mg.
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| Contaminents: |
Free of: HbsAg, Hepatitis B surface antigen and Hormones to HIV, Hepatitis C and HCV.
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| Protein content: |
Protein quantitation was carried out by two independent methods:
UV spectroscopy at 280 nm.Analysis by RP-HPLC, using a calibrated solution of hCG as a Reference Standard. |
| Usage: |
This material is offered for research, laboratory or further manufacturing purposes.
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