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Home > antibodies > Polyclonal Rabbit Anti SARS Nucleocapsid Protein - PRASARSSP
Polyclonal Rabbit Anti SARS Nucleocapsid Protein - PRASARSSP
| Source: |
Rabbit
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| Background: |
The spike (S) glycoprotein of coronaviruses mediates viral entry into host cells.Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. It is a type 1 viral fusion protein that characteristically contains two heptad repeat regions, denoted HR-N and HR-C, that form coiled-coil structures within the ectodomain of the protein. Previous studies have shown that the two heptad repeat regions can undergo a conformational change from their native state to a 6-helix bundle (trimer of dimers), which mediates fusion of viral and host cell membranes.
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| Description: |
Full-length Spike protein transfected into UM92 cells was used as a positive control and an approximate 139KDa band is detected.
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| Antigen: |
The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino acids 1124-1140 of the putative SARS Spike glycoprotein (Genbank accession no. NP_828851).
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| Isotype: |
Rabbit IgG
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| Formulation: |
0.5µg/µl PBS containing 0.2% gelatin and 0.05% sodium azide.
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| Storage: |
Store at 4C, stable for 6 months. For long-term storage, store at -20C
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| Applications |
Western blot SARS detection.
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| Usage: |
This material for research, laboratory or further evaluation purposes.
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| Latest Publications: |
Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody.
Chan PK, To WK, Ng TK, Houde M,
J Med Virol 2004 Dec;74(4):517-20
Antibody responses against SARS-coronavirus and its nucleocaspid in SARS patients.
Shi Y, Wan Z, Li P,
J Clin Virol 2004 Sep;31(1):66-8
[Determination and comparison of anti-SARS antibody in children and adults]
Liu JH, Ma SX, Wang HB, Tang SQ,
Zhongguo Shi Yan Xue Ye Xue Za Zhi 2004 Apr;12(2):217-9
[The quantitative detection of anti-coronavirus antibody titer in medical personnel closely contacted with severe acute respiratory syndrome patients]
Liu YN, Fan BX, Yu BX,
Zhonghua Jie He He Hu Xi Za Zhi 2003 Oct;26(10):583-5
[Detection of the anti-SARS-coronavirus specific antibody levels in 156 SARS patients]
Wang HB, Liu JH, Yu Y, Tian YP,
Zhongguo Shi Yan Xue Ye Xue Za Zhi 2003 Oct;11(5):441-3
[Primary investigation on the changing mode of plasma specific IgG antibody in SARS patients and their physicians and nurses]
Xu G, Lu H, Li Y, Zhao ZD,
Beijing Da Xue Xue Bao 2003 May 31;35 Suppl:23-5
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