| Immunogen |
Dengue-infected cell lysate |
| Ig Subclass |
Mouse IgG1/kappa |
| Purification Method: |
Protein A and Ion exchange column
|
| Protein Concentration: |
1 mg/ml in PBS (after reconstitution).
|
| Storage Procedure: |
Lyophilized: store at 4 C. After reconstitution, if not used within a month, aliquot and store at 20C.
|
| Reconstitution: |
Reconstitute with H20 to get a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
|
| Stability / Shelf life: |
Two years lyophilized, one month in solution at 4C.
|
| Titer: |
By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).
|
| NOTE: |
The antibody was produced in BALB/c mice. Will recognize FLAG conjugated at either the amino or carboxy terminus. Can be used bound to various resins for affinity purifications.
|