| Clone # |
ynrhTNF-a. |
| Immunogen |
r.Human TNF-a. |
| Ig Subclass |
Mouse IgG1. |
| Purification Method |
Protein A (high salt). |
| Protein Concentration |
1 mg/ml in PBS (after reconstitution). |
| Storage Procedures |
Lyophilized- store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. |
| Reconstitution |
Reconstitute with 0.5 ml of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Stability / Shelf Life |
Two years lyophilized, 1month in solution at 4°C. |
| Titer |
By direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson ImmunoResearch Laboratories) 1:10,000 dilution will yield 0.7 O.D within 10 minutes |
| Note |
This antibody will bind very well to protein A in a buffer (PBS) containing high salt concentration (3M Nacl). |