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Description |
Recombinant Human IGF-I produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7655 Dalton.
rHuIGF-I is purified by proprietary chromatographic techniques.
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Source |
Escherichia Coli. |
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Physical Appearance: |
Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation: |
The protein was lyophilized after extensive dialysis against 50mM acetic acid.
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Solubility: |
It is recommended to reconstitute the lyophilized rHuIGF-I in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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Stability: |
Lyophilized rHuIGF-I although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution rHuIGF-I should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
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Purity: |
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
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Amino acid sequence: |
The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu.
N-terminal methionine has been completely removed enzymatically.
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Dimers and aggregates: |
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
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Biological Activity: |
ProSpec's rHuIGF-I is fully biologically active when compared to standards.
The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is less then 1.0 ng/ml, corresponding to a specific activity of 1x106 U/mg.
For most in-vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20 ng/ml.
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Endotoxin: |
Less than 0.1 ng/µg (IEU/µg) of rHuIGF-I .
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Protein content: |
Protein quantitation was carried out by two independent methods:
UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction
coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE
computer analysis program of protein sequences (IntelliGenetics). Analysis by RP-HPLC, using a calibrated solution of IGF-I as a Reference Standard. |