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Description |
Recombinant Chicken IGF-I produced in E.Coli is a single, non-glycosylated,
polypeptide chain containing 70 amino acids and having a molecular mass of 7738 Dalton.
rcIGF-I is purified by proprietary chromatographic techniques. |
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Source |
Escherichia Coli. |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation |
The protein was lyophilized after extensive dialysis against 100mM acetic acid.
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Solubility |
It is recommended to reconstitute the lyophilized rcIGF-I in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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Stability |
Lyophilized rcIGF-I although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rcIGF-I should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
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Purity |
Greater than 95.0% as determined by:
Analysis by RP-HPLC.Anion-exchange FPLC.Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
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Dimers and aggregates: |
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
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Biological Activity: |
ProSpecs rcIGF-I is fully biologically active when compared to standards.
The ED50, was calculated 2 methods:
1. Stimulation of protein synthesis in rat L6 myoblasts
ED50 was found to be less then 30 ng/ml.
2. Type 1 IGF receptor binding assay ED50 was found to be less then 10 ng/ml. |
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Endotoxin: |
Less than 0.1 ng/µg (IEU/µg) of rcIGF-I.
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Protein content: |
Protein quantitation was carried out by two independent methods:
UV spectroscopy at 280 nm.nalysis by RP-HPLC, using a calibrated solution of chicken IGF-I as a Reference Standard. |
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Usage |
This material is offered for research, laboratory or further manufacturing purposes.
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Refrences |
Upton F.Z. et al. (1992) J. Mol. Endocrinol. 9, 83-92.
McMurtry J.P. et al. (1997) Dom. Animal Endocrinol. 14, 199-229.
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