| Description: |
Recombinant Murine IL-1 b produced in
E.Coli is a non-glycosylated, Polypeptide chain containing 153 amino acids
and having a molecular mass of 17500 Dalton. The rHuIL-1b is purified by
proprietary chromatographic techniques. |
| Source: |
Escherichia Coli. |
| Physical Appearance: |
Sterile Filtered White lyophilized
(freeze-dried) powder. |
| Formulation & packaging: |
Lyophilized from a concentrated
(1mg/ml) solution in water containing no additives |
| Solubility: |
The lyophilized rmIL-1b is very
soluble in water and most aqueous buffers below and above the isoelectric
point. |
| Stability: |
Lyophilized rmIL-1b although stable at
room temperature, should be stored desiccated below 0°C. Reconstituted
rmIL-1b is best stored refrigerated at 4°C. |
| Purity: |
Greater than 99.0% as determined by:
Analysis by RP-HPLC.Anion-exchange FPLC.Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
(Limit of acceptance:≥98.0%. No more than 2% total impurities; no single impurity greater than 1%)
|
| Amino Acid Composition: |
In total agreement with the expected
amino acid composition of native murine IL-1b.
|
| Amino acid sequence: |
The sequence of the first five
N-terminal amino acids was determined and was found to be
Met-Val-Pro-Ile-Arg. |
| Dimers and aggregates: |
Less than 1% as determined by
silver-stained SDS-PAGE gel analysis. |
| Biological Activity: |
murine IL-1b is fully
biologically active when compared to standard. The specific activity as
determined in the test of augmentation of lymphocyte prolifiration assay
using mouse thymus was found to be 6 x 108 / mg. |
| Endotoxin: |
Less than 0.1 ng/µg (IEU/µg) of murine
IL-1b. |
| Protein content: |
Protein quantitation was carried out by two independent methods:
UV spectroscopy at 280 nm.Analysis by RP-HPLC, using a calibrated solution of IL-1b as a Reference Standard. |
| Usage: |
This material is for research, laboratory
or further manufacturing purposes. |